QUESTIONS: (True/False or Yes/No)

1. Viral vectors may be added just like geometric vectors.
2. Viral envelopes may be very small or relatively large.
3. The size of the envelope determines how much DNA a virus can hold.
4. Helper molecules stop dangerous reactions
5. Luciferase is used as a genetic marker.
6. Viral vectors are being used for gene therapy.
7. We can alter any section of native DNA.
8. We can replace any section of native DNA with a foreign segment.
9. A virus is composed of a protein envelope and some viral genetic material.
10. Normally a virus contains the codes for building a duplicate virus.

QUESTIONS: (Multiple Choice)
1. Where do we get the viral envelopes that are used to make viral vectors?
a. Viral envelopes are found in nature
b. Viral envelopes are synthesized
c. From infected chickens and other animals
d. They are cultured in a laboratory
e. All of the above

2. Is there a danger of creating a new and deadly virus by working with viral vectors?
a. Yes
b. No
c. The second law of thermodynamics prevents that from happening
d. There is always a small danger
e. There is always a great danger

3. Once a segment of DNA is introduced into a living cell, the cell...
a. Attacks the foreign DNA with its immune response
b. Digests the DNA with its enzymes
c. Permits only limited replication of this DNA
d. Dies
e. Treats the foreign DNA as native DNA

4. Alignment occurs whenever...
a. Two cells are next to each other
b. A viral vector introduces some DNA into a cell
c. A virus attaches its envelop to a cell membrane
d. Matching segments of DNA line up next to each other
e. The bases A and T or G and C are paired

5. Soon there may be a molecular library that contains all the codes for every human gene. With such a library we could...
a. Cure every known disease
b. Cure every known and still unknown disease
c. Cure only genetic disorders
d. Create new genetic disorders
e. c and d

QUESTIONS: (Essay)
1. Why would a viral vector work better than a vaccine in the bloodstream to combat a genetic disorder?
2. Describe the alignment of segments of viral introduced DNA with native DNA.
3. If we can alter the human genome with ease, what changes, if any, should we make?
4. Treatment of a genetic defect causing cystic fibrosis has been carried out by a common cold viral vector. Unfortunately, once the cold has passed, the disease returns. What are the implications of this short-lived therapy?
5. Describe how a marker such as the sensitivity to an antibiotic or the production of luciferase may be used to track the progress of some genetic reactions.