QUESTIONS: (True/False or Yes/No)

1. A blastomere is a tiny ball of embryonic cells.
2. Each cell in a blastomere is genetically distinct from its neighbor.
3. Animals can not be cloned.
4. Humans can not be cloned.
5. Nuclear transplantation involves isotopes.
6. Developing cells need cytoplasm.
7. Identical twins are natural clones.
8. Rabbits can carry cow embryos.
9. "Bovine" means pig.
10. Once the nucleus is removed from an oocyte that oocyte has no DNA.

QUESTIONS: (Multiple Choice)

1. A blastomere can contain _______ cells.
a. 7 b. 13 c. 16 d. 19 e. 22
2. Which are steps in the cloning process?
a. harvesting blastomere cells
b. electrofusion of cell membranes
c. nuclear transfer
d. harvesting oocytes
e. discarding DNA
1. a,b,c,d
2. c,d,e
3. a,b,c,e
4. none of the above
5. all of the above

3. Why clone farm animals?
a. it's cheaper than breeding
b. it's faster than breeding
c. you always know what you get
d. prized animals are worth a lot of money
e. they have smaller oocytes than humans
1. a,b,c,d
2. a,b,d,e
3. b,c,d
4. none of the above
5. all of the above

4. Part of the cloning process involves transferring a nucleus from a blastomere cell into an oocyte. This is done because:
a. the nucleus needs nutrients
b. the oocyte needs protein
c. the nucleus can't survive without cytoplasm
d. the oocyte cell membrane contains luciferase
e. the nucleus and oocyte will form one cell
1. a,b,c
2. a,b,d
3. a,b,c,e
4. none of the above
5. all of the above

5. The DNA (the genome) of a single blastomere cell is...
a. different from each of its neighboring cells
b. identical to each of its neighboring cells
c. is going to degrade after nuclear transfer
d. will be replaced by oocyte DNA
e. contains the plans to build an entire organism
1. a,b,c
2. b,e
3. c,e
4. none of the above
5. all of the above

QUESTIONS: (Essay)

1. Describe at least three major steps of the cloning process.
2. What are some of the controversies surrounding the cloning of humans?
3. Why is cloning better than traditional breeding for the developing breeds of animals?
4. Describe some of the problems or limitations of the cloning process.
5. After nuclear transfer why is an electric shock required in the cloning process? Explain both reasons.